TABLE 1.
Primers used in this study
| Name | Sequence (5′ to 3′) |
|---|---|
| ltnA1A2SOEA | AACTGCAGTTATATATTTGCGGCa |
| ltnA1A2SOEB | GATATGAACCTCCTTATT |
| ltnA1A2SOEC | AATAAGGAGGTTCATATCTTAGGAGTAAAAAATGAAb |
| ltnA1A2SOED | ACGAATTCTCTTACAGAGTTc |
| ltnA1SOEC | AATAAGGAGGTTCATATCGTTAATAACAAATTTTTAATTAAT |
| ltnA1SOED | TGTCGAATTCATTTCTGGAAAAACc |
| pORI280 For | CTCGTTCATTATAACCCTC |
| pORI280 Rev | CGCTTCCTTTCCCCCCAT |
a
Boldface represents changes made to incorporate a PstI site.
b
Underlined sequence represents an overhang that is the reverse complement of the corresponding SOEB primer.
c
Boldface represents changes made to incorporate an EcoRI site.