TABLE 2.
Sequencing of PCR amplicons derived from isolated cell clonesa
| Sample | Cell clone | Sequence length (bp) | Ambiguity
|
Most closely related species (% sequence similarity)c | |
|---|---|---|---|---|---|
| bp | % | ||||
| B1, M1b | MBRG 1.5 | 750 | 0 | 0 | B. subtilis AJ276351 (99) |
| B1, M1 | MBRG 1.6 | 696 | 8 | 1.1 | E. asburiae AB004744 (99) |
| B1, M1 | MBRG 1.2 | 742 | 7 | 0.9 | H. alvei M59155 (99%) |
| B1, M1 | MBRG 1.5 | 757 | 16 | 2.1 | K. oxytoca AB053117 (99) |
| B1, M1 | MBRG 1.4 | 798 | 15 | 1.9 | L. paracasei AF243147 (97) |
| B1, M1, B2 | MBRG 1.7 | 756 | 2 | 0.3 | Pseudomonas sp. strain P400Y AB076857 (99) |
| B1, M1 | MBRG 1.1 | 772 | 6 | 0.8 | K. planticola AF129443 (99) |
| B2 | MBRG 2.4 | 760 | 2 | 0.3 | B. subtilis Z99104 (99) |
| B2 | MBRG 2.3 | 744 | 0 | 0 | M. osloensis X95304 (99) |
| B2 | MBRG 2.1 | 771 | 5 | 0.6 | E. coli U18997 (99) |
| B2 | MBRG 2.5 | 785 | 4 | 0.5 | S. epidermidis AF269927 (99) |
| B3 | MBRG 3.1 | 739 | 4 | 0.5 | E. coli AE000406 (98) |
a
Compared to published sequences, according to BLAST searches.
b
Amplicons derived from both inoculum (B1) and microcosm (M1).
c
Percent similarity values based on pairwise alignments.