TABLE 3.
Sequencing of PCR amplicons (dominant phylotypes) derived from DGGE gelsa
| Sample | Cell clone | Sequence length (bp) | Ambiguity
|
Most closely related species (% sequence similarity)c | |
|---|---|---|---|---|---|
| bp | % | ||||
| B1 | B1a/M1b | 181 | 19 | 10.5 | K. oxytoca AB004754 (80) |
| B1, M1b | B1b/M1c | 183 | 37 | 20.2 | K. oxytoca AB004754 (72) |
| M1 | M1a | 180 | 31 | 17.2 | S. multivorum AB020205 (72) |
| B2 | B2a | 182 | 10 | 5.5 | M. osloensis AF00519016S (94) |
| B2 | B2b | 185 | 12 | 6.5 | M. osloensis AJ269515 (89) |
| B3 | B3a | 183 | 13 | 7.1 | E. coli AF076037 (92) |
| B3 | B3b | 181 | 10 | 5.5 | E. coli AF076037 (91) |
| B4, M4 | B4a/M4b | 184 | 42 | 22.9 | Chryseobacterium sp. strain AJ309324 (62) |
| B4, M4 | B4b/M4b | 177 | 38 | 21.5 | Chryseobacterium defluvium AJ309324 (65) |
| M4 | M4c | 180 | 23 | 12.8 | P. dentalis X81876 (81) |
a
Compared to published sequences, according to BLAST searches.
b
Amplicons derived from both inoculum (B1) and microcosm (M1).
c
Percent similarity values based on pairwise alignments.