TABLE 1.

Sequences of oligonucleotides used as PCR primers and for FISH with corresponding concentrations of formamide and NaCl

Probe or primer	Sequence (5′-3′)	Target organisms	% Formamide in hybridization buffer	Concn of NaCl in washing buffer (mM)	Reference
EUB338	GCTGCCTCCCGTAGGAGT	Bacteria	0	900	1
ARC915	GTGCTCCCCCGCCAATTCCT	Archaea	20	225	53
MS821	CGCCATGCCTGACACCTAGCGAGC	Methanosarcina	30	112	45
MSMX860	GGCTCGCTTCACGGCTTCCCT	Methanosarcinales	30	112	45
NONa	ACTCCTACGGGAGGCAGC	NAb	NA	NA	32
Arch21Fa	TCCGGTTGATCCYGSCRGc	Archaea	NA	NA	9
ARC344Fa-Clamp	CGCCCGCCGCGCCCCGCGCCCGTCCCGCCGCCCCCGCCCG-ACGGGGYGCAGCAGc	Archaea	NA	NA	45
GM4R	TACCTTGTTACGACTT	All organisms	NA	NA	40
341F-Clamp	CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGG-CCTACGGGAGGCAGCAG	Bacteria	NA	NA	39
907R	CCGTCAATTCMTTTGAGTTT	Bacteria	NA	NA	39

^aUsed as negative control.

^bNA, not applicable.

^cSequence modified from original publication.