FIG. 2.

Cytometric analysis of DiBAC₄(3)-derived green fluorescence of log-phase growing cells and bacterial ghosts of E. coli NM522(pML1). Ghosts or cells were incubated separately with DiBAC₄(3) at concentrations ranging from 0.01 to 5 μM. Cytometric analysis was performed immediately (gray bars) after dye addition or after an incubation period of 1 (bars with thin black stripes), 5 (black bars), 10 (white bars), or 20 (bars with thin white stripes) min. For each individual analysis, the fluorescence of 10⁴ ghosts or cells was quantified. The dye concentration is given in micromolar units, whereas the mean values of green fluorescence are presented in arbitrary units (a.u.).