FIG. 1.

In situ hybridization of ectosymbiotic spirochetes of gut protists stained simultaneously with the consensus probes for cluster I and cluster II. The protists D. porteri in R. speratus (A1 to A4) and Pyrsonympha sp. in H. sjoestedti (B1 to B4) are shown. The cluster II consensus probes TT-484V3 and TT-1248V8, labeled with 6-FAM (A1 and B1), and the cluster I consensus probe TTI-732, labeled with Texas Red (A2 and B2), were used. Confocal laser scanning microscopy visualized the fluorescent signals. (A3 and B3) Differential interference contrast micrographs. Bars, 10 μm. (A4) Merged image of panels A1 and A2; (B4) merged image of panels B1 and B2.