TABLE 1.
Sorting of spirochete 16S rDNA clones from isolated protist cells into phylotypes
| Isolated protist | No. of clones analyzed | No. of phylotypes | Representative clonea (no. of clonesb) | Identical phylotype from whole gutc |
|---|---|---|---|---|
| Devescovina sp. | 31 | 2 | NkS-Dev1 (28) | NkS93 |
| NkS-Dev14 (3) | NkS4 | |||
| Stephanonympha sp. | 25 | 3 | NkS-Ste2 (15) | NkS4 |
| NkS-Ste1 (6) | NkS93 | |||
| NkS-Ste9 (4) | (NkS56)d | |||
| Oxymonas sp. | 27 | 6 | NkS-Oxy8 (11) | NkS21 |
| NkS-Oxy26 (7) | NkS56 | |||
| NkS-Oxy1 (4) | −e | |||
| NkS-Oxy3 (2) | NkS97 | |||
| NkS-Oxy25 (2) | NkS9 | |||
| NkS-Oxy70 (1) | NkS4 |
a
Representative clones were included in the phylogenetic analysis shown in Fig. 3.
b
Number of clones that were grouped into the same phylotype as the representative clone.
c
The identical phylotype that was identified in the spirochete 16S rDNA libraries from the whole gut community of N. koshunensis.
d
The clone NkS-Ste9 had no identical phylotypes in the libraries from the whole gut community but was closely related (96.1% nucleotide identity) to the clone NkS56.
e
The clone NkS-Oxy1 had no close relatives in the libraries from the whole gut community.