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Disruption of ZbACS2 (accession number AJ314837) by APT1. (A) Schematic representation of the APT1 expression cassette for disruption experiments. (B) Southern blot of the Z. bailii ISA 1307 wild-type strain (lanes 1 and 3) and mutant 14, which is resistant to Geneticin (Z. bailii ISA 1307-14) (lanes 2 and 4). The genomic DNA isolated was digested with the restriction enzyme HindIII (lanes 1 and 2) or PstI-XhoI (lanes 3 and 4).
