TABLE 1.
Primer | Sequencea |
---|---|
NC8-4 | 5′-TGRTARAAICCYTTRTTRAA-3′ |
NC8-6 | 5′-GGIATHAARATHYTITGG-3′ |
NC8-7b | 5′-GGTCTGCGTATAAGCATCGC-3′ |
M13(−20) | 5′-GTAAAACGACGGCCAGT-3′ |
M13R(−48) | 5′-AGCGGATAACAATTTCACACAGGA-3′ |
I, deoxyinosine; R, A or G; Y, C or T; N, A, C, G or T; H, A, C, T.
NC8-7 was also used as a probe for Southern hybridization after tailing at the 3′ end with fluorescein-11-dUTP.