TABLE 2.

Primers used in this study

Primer	Sequencea	Positionb	Source or reference
L. pseudomesenteroides
BO1	5′-AAG TTG CAA TGG TTA CCN G-3′	708→	This work
BO2	5′-ACA ACG TAC AGC GAA CTT-3′	1183←	This work
CDR1	5′-GCA AGC GCG CAT GTG GAT C-3′	20→	This work
CDR2	5′-CTT GTT GCA ATA TCA TGA TTG GC-3′	2182←	This work
CDR3	5′-GAC TAT AGT TGT TTC GAA TAA TAC-3′	657→	This work
CDR10	5′-ATA TAT GAA TTC GCA AGC GCG CAT GTG GAT C-3′	20→	This work
CDR11	5′-ATA TAT GAA TTC CTT GTT GCA ATA TCA TGA TTG GC-3′	2182←	This work
CDR15	5′-ATT TTT AGT TAA TTA TAC TAA TTC CTC CAA TAG TAT TAT TCG AAA C-3′	697←	This work
CDR16	5′-TTG GAG GAA TTA GTA TAA TTA ACT AAA AAT AAT TAA CTT TTA CCC-3′	1469→	This work
Bos taurus
RT-DR1	5′-GGG TTG AAG TTG TCC ATG CGA-3′	→	29
RT-DR2	5′-CAA ATC AGC AAC TGC AAC TGC-3′	←	29

^aUnderlined nucleotides signify EcoRI restriction sites. For CDR 15, nucleotides in bold correspond to a 15-bp sequence with homology at the 5′ end of a 741-bp fragment downstream of the coding region of butA; for CDR 16, nucleotides in bold correspond to a 15-bp sequence with homology at the 3′ end of a 705-bp fragment upstream of the coding region of butA.

^bFor L. pseudomesenteroides primers, numbering is based on GenBank accession no. AF438200. Arrows indicate the orientation.