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Induction and cellular location of chlorogenate esterase detected as HcaG3::T7 · Tag in cell extracts (A and B) and in a membrane preparation (C). The amount of protein separated by SDS-PAGE in each lane is indicated. Immunoblots show the presence of the esterase in extracts of cells grown with chlorogenate, caffeate, ferulate, and coumarate (A and B) but not in extracts of cells grown with quinate (A). The esterase was associated with the membrane fraction of cell extracts (C).
