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(A) Schematic representation of the organization of the DNA fragment amplified by PCR with primers OD1 and OD2 in order to sequence the ctc gene of L. monocytogenes LO28. The solid bar represents the DNA fragment amplified by PCR with primers OD3 and OD4 and subsequently cloned into the pAUL-A plasmid in order to interrupt the ctc gene. Arrows indicate the orientations of the genes. (B) RNA slot blot transcription analysis of the ctc gene.
