FIG. 3.

RT-PCR of insertion regions. RNA was isolated from strain 10403 for RT-PCR. Primers used to determine the cotranslation of insertions Ω001 and Ω002 were orf2for and orf5rev (lanes 1 and 2), and the expected product was 2.745 kb. Primers used to determine the cotranslation of insertion Ω003 and the gene downstream of it were Ω003for and Ω003rev (lanes 3 and 4), and the expected product was 1.7 kb. The locations of the primers on the chromosome are indicated by asterisks in Fig. 2. Lanes 2 and 4 are controls that contained no RT to test the purity of the RNA preparations. Size markers are in kilobases.