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. 2003 Jan;69(1):358–366. doi: 10.1128/AEM.69.1.358-366.2003

FIG. 7.

FIG. 7.

SDS-PAGE analysis of the active-form MTG produced by C. glutamicum carrying plasmids expressing pro-MTG and SAM-P45. Ten microliters of supernatant and an equal volume of sample buffer were applied to each slot and analyzed by SDS-PAGE. After electrophoresis, the gel was stained with SYPRO Orange as described in Materials and Methods. Lane 1, molecular weight markers; lanes 2, 3, 4, 5, 6, and 7, culture supernatants after 24, 30, 45, 54, 70, and 140 h of cultivation, respectively; lane 8, purified MTG from S. mobaraense.