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Cell surface transport of GP-C N-glycosylation mutants. Vero cells were transfected with GP-1 (A) and GP-2 (B) N-glycosylation mutants of Lassa virus glycoprotein. At 24 h post-transfection, cells were surface labelled with biotin. Following cell lysis and immunoprecipitation using α-GP-2, samples were subjected to SDS-PAGE and blotted to nitrocellulose. Surface biotin-labelled proteins were visualized with streptavidin-peroxidase and chemiluminescence.
