FIG. 5.

Assessment of created strains by biphenyl degradation activity. Strains were cultivated in 1/3 LB medium to the stationary phase. The portions of each culture were transferred to test tubes. To the tubes, biphenyl was added at a final concentration of 250 mg/liter. The tubes were incubated at room temperature with vigorous shaking. At several time points, ethyl acetate was added to the tube and residual biphenyl was extracted. The amount of biphenyl in the ethyl acetate was analyzed by GC-MS. The cell-free 1/3 LB medium was used as a control. (A) Cells and biphenyl were reacted for 30, 60, and 120 min. The values are the averages of two parallel samples. (B) Cells and biphenyl were incubated for 15 min. Each bar represents the average of four parallel experiments, and vertical lines represent standard deviations.