Table 1.

The protocol for hormone administration of none, one or two primings for female Bufo baxteri and the hormone type and concentration. No females ovulated when administered a single normally ovulatory hormone dose (500 IU hCG plus 4 micrograms of LHRHa) in the absence of priming (No priming). Nor did females ovulate when administered only an anovulatory hormone dose (100 IU hCG and 0.8 micrograms of LHRHa; One priming), or the normally inducing ovulatory dose and then after 72 hrs the anovulatory dose (Two primings). Females receiving both one and two primings were given thier anovulatory dose 96 hrs before the final ovulating dose resulting in a total time of 168 hrs for females receiving two primings.

		Hormone concentration
Treatment	Priming dose	Priming dose	Ovulatory dose	Result
Time (hours)	0 hrs	72 hrs	168 hrs
No priming	500 IU hCG + 4 μg LHRHa			No ovulation
One priming		100 IU hCG + 0.8 μg LHRHa	500 IU hCG + 4 μg LHRHa	Ovulation
Two primings	500 IU hCG + 4 μg LHRHa	100 IU hCG + 0.8 μg LHRHa	500 IU hCG + 4 μg LHRHa	Ovulation