Figure 4.

Immunoblotting of LARG and RhoA. The LARG expression level was decreased under altered gravity, whereas the expression level of RhoA was higher in the clinorotated cells than in the control cells. Cell lysis and immunocomplex detection of LARG, RhoA, and GAPDH were performed as described in the Methods section. In panel A, lanes C and L indicate the lysates prepared from the stationary control cells and the clinorotated cells, respectively. Each lysate contains 10 μg of total proteins. Panel B shows the density of the western blots in panel A quantified by the Quantity One software (Bio-Rad). The data shown are representative of three individual experiments. Values are represented as means ± SE (p < 0.05).