Figure 5.

Rho activity assay. The active form of RhoA was luminometrically detected using a G-LISA kit. RhoA activity decreased significantly in the clinorotated cells. The lysate was extracted from cells that were cultured under 3D clinostat for 72 h. The lysate including 60 μg of total proteins was immediately reacted with a reaction reagent included in the kit. Values were obtained from eight separate experiments performed in triplicates and are represented as means ± SE (p < 0.05, control vs. clinorotation). His-RhoA (Q63L), a constitutively active RhoA protein containing a glutamine to leucine substitution at residue 63 was used as positive control in the G-LISA reaction.