Table 2.
Chemokine receptor expression in memory T helper cells induced by 7 days of antigenic stimuli. Median chemokine receptor expression in memory Th cells in percentage and MFI after 7 days of stimulation with antigen (allergen (15 μg/ml) or TTx (10 μg/ml)) or no antigen as a control. n = 10 for the healthy controls, n = 10 for the asymptomatically sensitized individuals and n = 8 for the allergic individuals except for CCR8 where n = 6 for the asymptomatically sensitized and allergic individuals. 10.000 PBMCs were acquired for the analysis. Isotype control cut-off values were set to >98%. Samples were run in monocates. For experimental design and analysis see Methods.
| % median (range) | MFI median (range) | ||||||
| No Ag | Allergen | TTx | No Ag | Allergen | TTx | ||
| CCR3 | Allergic | 10.6 (4.7–32) | 17.3 (9.8–29.5) | 15.6 (4.4–52.2) | 21 (16.2–32.3) | 19.4 (16.9–37.2) | 26.1* (16.6–40.5) |
| AS | 10.3 (3.3–19.7) | 8.7 (2.8–23.2) | 11.9 (5–28.8) | 20.5 (14.7–27.2) | 18.6 (16.4–32.3) | 23.4 * (18.4–37.4) | |
| Healthy | 8.7 (1.5–45) | 11.2 (3.2–36.8) | 23.4 (4.5–44.3) | 19.7 (12.6–45.4) | 17.8 (12.5–32.2) | 23.7 (12.5–38.4) | |
| CCR5 | Allergic | 21.3 (7.8–31.5) | 25 (4.1–53.9) | 28.3 (12.3–61.1) | 21.4 (16.8–27.9) | 25.9 * (22.4–48.5) | 26.1 (16.1–42.5) |
| AS | 15.2 (2.6–36.6) | 17 (3–33.5) | 16.9 * (5.2–65.9) | 18.3 (15.2–24.5) | 17.5 (15.4–20.5) | 22.5 * (18.2–58.2) | |
| Healthy | 11.7 (5.6–34.5) | 13.5 * (6.7–29.7) | 20.2 * (10.1–74.1) | 16.7 (12.7–22) | 17.3 (13.5–25.6) | 25.1 * (13.4–72) | |
| CCR8 | Allergic | 10.4 (1.6–20.4) | 12.8 (3.3–20) | 12.7 (2.2–27.4) | 24.3 (16.8–37.6) | 27.9 (18.8–40.3) | 28.2 (17.8–82.6) |
| AS | 3.7 (1.8–16.6) | 3.2 (1.7–14.7) | 6.3 (1.4–34.9) | 21.9 (17–26.1) | 22.8 (17.2–44.6) | 24.9 (16.2–36.6) | |
| Healthy | 5.7 (0.7–16.4) | 3.9 (1.5–14) | 9.3 * (3.8–35.7) | 19 (12.6–49.6) | 19.2 (11.7–46.6) | 23.9 (12.4–48.2) | |
| CXCR3 | Allergic | 42.9 (22–47.5) | 42.1 (19.2–67.4) | 49.2 * (27–74.2) | 57.4 (33.1–65.3) | 54.4 (48.4–72.4) | 60.6 (36.9–81.8) |
| AS | 28.1 (18–56.6) | 27.8 (21–56.8) | 29 * (20.8–77.4) | 39.7 (29.3–59.7) | 42.3 (30.6–54.5) | 46.5 (33.5–115) | |
| Healthy | 28.8 (19.8–46.5) | 31.4 (17.3–46.9) | 35.9 * (25.8–82.9) | 37.8 (32–65.2) | 39.3 (30.1–63.9) | 57.6 * (32–162.4) | |
Bold text and * indicates significant differences between the antigen (allergen or TTx) stimulated samples and control samples where no antigen was added.
Ag: antigen AS: asymptomatically sensitized individuals.