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PCR-OLA identification of quinolone resistance mutations in parC of S. pneumoniae. Shown is the 96-well colorimetric reaction for several strains of S. pneumoniae. Included were triplicate samples of strains with wild-type (WT) ParC (Asp83) and ParC with Asp83-Tyr, Asp83-Asn, and Asp83-His mutations. Top row, positive control for Asp83-Asn mutation; second row, positive control for WT Asp83; third to eighth rows, various strains of S. pneumoniae.
