TABLE 1.
Protein | Position | Target amino acidb | Codon | Ligation probea
|
|
---|---|---|---|---|---|
Capture | Reporter | ||||
ParC | 79 | Ser | TCT | TTCCACCCACACGGGGATTC | TTCTATCTATGATGCCATGGTTC |
Tyr | TAT | TTCCACCCACACGGGGATTA | TTCTATCTATGATGCCATGGTTC | ||
Phe | TTT | TTCCACCCACACGGGGATTT | TTCTATCTATGATGCCATGGTTC | ||
Ala | GCT | TTCCACCCACACGGGGATGC | TTCTATCTATGATGCCATGGTTC | ||
83 | Asp | GAT | CGGGGATTCTTCTATCTATG | ATGCCATGGTTCGTATGTCT | |
Tyr | TAT | CGGGGATTCTTCTATCTATT | ATGCCATGGTTCGTATGTCT | ||
Asn | AAT | CGGGGATTCTTCTATCTATA | ATGCCATGGTTCGTATGTCT | ||
His | CAT | CGGGGATTCTTCTATCTATC | ATGCCATGGTTCGTATGTCT | ||
Gly | GGT | CGGGGATTCTTCTATCTATG | GTGCCATGGTTCGTATGTCT | ||
Val | GTT | CGGGGATTCTTCTATCTATG | TTGCCATGGTTCGTATGTCT | ||
GyrA | 84 | Ser | TCC | TATCACCCACACGGGGATTC | CTCTATTTATGAAGCCATGGTCC |
Phe | TTC | TATCACCCACACGGGGATTT | CTCTATTTATGAAGCCATGGTCC | ||
Ala | GCC | TATCACCCACACGGGGATGC | TCTATTTATGAAGCCATGGTCC | ||
Tyr | TAC | TATCACCCACACGGGGATTA | TCTATTTATGAAGCCATGGTCC | ||
Cys | TGC | TATCACCCACACGGGGATTG | TCTATTTATGAAGCCATGGTCC | ||
88 | Glu | GAA | ACACGGGGATTACTCTATTTATG | AAGCCATGGTCCGTATGGCT | |
Lys | AAA | ACACGGGGATTACTCTATTTATA | AAGCCATGGTCCGTATGGCT | ||
Gly | GGA | ACACGGGGATTACTCTATTTATG | GAGCCATGGTCCGTATGGCT |
Capture probes are labeled with biotin on the 5′ end. Reporter probes are phosphorylated on the 5′ end and labeled with digoxigenin on the 3′ end.
For each position, the wild-type amino acid is given first.