TABLE 2.

Ciprofloxacin MICs and OLA results for 53 strains of S. pneumoniae

No. of strains	Ciprofloxacin MIC (μg/ml)	Amino acid substitutionb at indicated position in:				Resulta
		ParC		GyrA
		79	83	81	85
5	0.5	+	+	+	+	Yes
5	1	+	+	+	+	Yes
6	2	+	+	+	+	Yes
4	4	+	+	+	+	Yes
2	4	Phe	+	+	+	Yes
1	4	+	Asn	+	+	Yes
2	8	+	+	+	+	Yes
1	8	Phe	+	+	+	Yes
1	8	Phe	+	Phe	+	Yes
1	8	+	Asn	+	+	Yes
1	16	+	+	+	+	Yes
1	16	Phe	+	Phe	+	Yes
1	16	Phe	+	+	Lys	Yes
1	16	Tyr	+	Ala	+	Yes
1	16	+	Tyr	Phe	+	Yes
3	16	Tyr	+	Phe	+	Yes
1	16	Tyr	+	Tyr	+	Yes
1	16	Tyr	+	+	+	Yes
1	32	+	+	Phe	+	Yes
1	32	Tyr	+	Phe	+	Yes
1	32	Tyr	+	+	Lys	Yes
3	32	Phe	+	Phe	+	Yes
1	32	Tyr	+	+	Lys	Yes
1	32	+	Tyr	Phe	+	Yes
1	32	Tyr	+	Tyr	+	Yes
2	64	Phe	+	Phe	+	Yes
1	64	Phe	+	Tyr	+	Yes
1	64	Tyr	+	Phe	+	Yes
1	64	Tyr	Val	Phe	+	Yes
1	64	+	Asn	Tyr	+	Yes

^aYes, PCR-OLA in agreement with sequence results.

^b+, wild-type amino acid.