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Amounts of MexY produced by strain PAO1 after a single gentamicin exposure at 1.6 mg/liter (MIC; lane 1a), 0.8 mg/liter (half the MIC; lane 2a), or 0.4 mg/liter (one-fourth the MIC; lane 3a) for 2 h. Western blots were prepared as described in the legend for Fig. 2 and developed with a MexY antiserum. In each case, exposed bacteria were subsequently cultured for 24 h in drug-free MHB (lanes 1b, 2b, and 3b).
