TABLE 5.
MICs, catalase activity, and mutations in katG for INH-resistant M. tuberculosis isolatesa
| Isolate | katG mutationb | Catalase (activity height of bubbles [mm])c | MIC (μg/ml) |
|---|---|---|---|
| UT450 | S315I | 13 | 32 |
| UT473 | W397Y, N529D | 15 | >256 |
| UT561 | S315T | 26 | 1 |
| UT739 | S315T | 10 | 4 |
| UT1885 | S315T | 25 | 12 |
| UT1998 | S315T | 15 | 0.75 |
| UT2007 | S315T | 15 | 6 |
| UT2090 | 124; G Ins | 5 | >256 |
| UT2204 | L91R | 5 | 2 |
| UT2340 | S315T | 20 | 0.38 |
| UT2419 | S315T | 20 | 4 |
| UT2883 | S315T | 10 | 8 |
| UT2931 | S315T | 25 | 0.5 |
| UT3171 | W90STOP | No bubbles | >256 |
| UT3174 | Del | No bubbles | >256 |
| UT3181 | Del | No bubbles | >256 |
| UT3194 | S315T | 14 | 19 |
| UT3306 | Q434STOP | 5 | >256 |
| HN1984 | S315N | 5 | >256 |
| HN1993 | A110V | 10 | 0.19 |
| NHN670 | A110V | 9 | 1.5 |
| NHN673 | A110V | 10 | 0.25 |
| NHN674 | A110V | 10 | <0.016 |
| H37Rv | WT | 25 | <0.016 |
a
H37Rv was used as a positive control.
b
The single-letter amino acid designations used are as follows: A, alanine; D, aspartic acid; I, isoleucine; L, leucine; N, asparagine; P, proline; Q, glutamine; R, arginine; S, serine; T, threonine; V, valine; W, tryptophan; and Y, tyrosine. The single-letter nucleotide designation used is as follows: G, guanine. The other abbreviations are as follows: Ins, insertion of bases; Del, deletion of the gene; STOP, termination mutation; WT, wild type.
c
No bubbles indicates a lack of catalase activity. Only isolates with data on catalase activity are shown.