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. 2006 Aug;12(8):1569–1582. doi: 10.1261/rna.39806

FIGURE 3.

FIGURE 3.

Stabilization of SSAT-X mRNA with protein synthesis inhibitors. (A) Syngenic or MT-SSAT transgenic fetal fibroblasts were exposed to cycloheximide (10 μg/mL [CHX1] or 50 ng/mL [CHX2]) for 6 h, pretreated with or without 10 μM DENSpm (added 24 h before CHX). (B) The cells were exposed to puromycin (100 μg/mL [PUR1] or 200 ng/mL [PUR2]) for 6 h with or without 10 μM DENSpm (added 24 h before PUR). Total RNA was extracted and analyzed with SSAT-X-specific primers (P3/P4). Lanes represent individual samples. The relative amount of SSAT-X (SSAT-X/total SSAT) was quantified with qPCR. n = 2, analyzed in triplicate.