FIGURE 5.

Stabilization of SSAT-X mRNA after siRNA-mediated silencing of Upf1 protein. MT-SSAT transgenic fetal fibroblasts were electroporated with negative control or Upf1 siRNA. Ten micromolar DENSpm was added after 24 h and the cells were harvested 72 h after electroporation. Silencing of Upf1 protein was detected from cell lysates by immunoblotting (WB) with anti-Upf1 antibody and anti-cyclophilin (CP) as a control. RT-PCR was carried out with primers P1 and P2. The relative amount of SSAT-X (SSAT-X/total SSAT) was quantified with qPCR. Values are means ± SD (n = 3, analyzed in triplicate). **p < 0.01, ***p < 0.001 compared to negative control group.