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Polyamine depletion increases the relative amount of SSAT-X variant. Wild-type or spermine-deficient embryonic stem cells were treated with 20 μM DENSpm or 1 mM DFMO supplemented with or without 100 μM Me2Spm for 48 h. Quantitative PCR data for SSAT-X expression (means ± SD, n = 3, analyzed in triplicate) are presented as relative to the amount of total SSAT mRNA. **p < 0.01, ***p < 0.001 compared to untreated control group.
