FIGURE 7.

Expression of PTC-containing splice variants in syngenic and MT-SSAT transgenic mouse fetal fibroblasts. (A) Cdc2-like kinase 1 (Clk1), (B) SC35, (C) rpL3, (D) rpL12, (E) 18S rRNA. RT-PCR shows both productive and PTC-containing unproductive variant of Clk1 and SC35. For rpL3 and rpL12, unproductive variant-specific primers were used. cDNA samples from previous experiments (Fig. 4B, Table 4) were used for RT-PCR, and the relative amounts were analyzed by densitometry with ImageQuant software. Values were normalized to 18S rRNA control values. D = DENSpm; B = Me₂Spm.