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The branched conformation of MD-III-2 RNA is less stable than the unbranched rod conformation. Upper and Lower bands were isolated from a nondenaturing gel. Purified RNA was diluted in water and incubated at 40°C. Aliquots were removed and placed on ice at indicated time points, then electrophoresed on a nondenaturing 6% polyacrylamide gel. Arrows indicate migrations of branched and unbranched RNAs.
