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. 2006 Aug;12(8):1505–1513. doi: 10.1261/rna.2321606

FIGURE 2.

FIGURE 2.

Back-to-back comparison of the transcription-stimulatory activity of S1 and RapA. Reactions with ptac1617 (Sukhodolets et al. 2001) (A) and pCPGλtr2 (B) supercoiled DNA templates were carried out in the presence of 2.1 μM S1 and 2.4 μM RapA. RNA polymerase/DNA template ratio and concentrations and conditions for in vitro transcription reactions were as described earlier (Sukhodolets et al. 2001). Promoter-specific transcripts resulting from in vitro transcription reactions carried out in the presence of 100 mM NaCl (top) and 200 mM NaCl (bottom) are shown.