Skip to main content
. 2006 Aug;12(8):1514–1520. doi: 10.1261/rna.101606

FIGURE 2.

FIGURE 2.

Dcr-2 stabilizes R2D2 in vivo, and R2D2 is required for RNAi. (A) Lysates were prepared from wild-type (lane 1) and homozygous r2d21 (lane 2) or dcr-2R416X (lane 3) flies. The levels of Dcr-1, Dcr-2, R2D2, and Ras proteins were measured by Western blots with the corresponding antibodies (Dcr-1/Dcr-2 or R2D2/Ras used the same blot). (B) The dsRNA-processing assays were performed in buffer alone (lane 1) or with 8 μg wild-type (lane 2), r2d21 (lane 3), and dcr-2R416X (lane 4) whole fly lysates. (C) The dsRNA-initiated RISC assays were performed in buffer alone (lane 1) or 32-μg ovary lysates prepared from wild-type (lane 2), r2d21 (lane 3), dcr-2R416X (lane 4), and r2d21 mutant flies carrying the rescue transgene (lane 5).