FIGURE 4.

Effect of Paip2B on in vitro translation of capped and polyadenylated bicistronic CAT-HCV IRES-luciferase mRNA. Capped and polyadenylated bicistronic CAT-HCV IRES-luciferase mRNA was translated by using Krebs-2 cell-free translation reactions in the presence of increasing concentrations of GST (control), GST-Paip2B, or GST-Paip2A. (A) First cistron, cap-dependent, and poly(A)-dependent translation. (B) Second cistron, cap-independent, and poly(A)-independent translation. (C) First and second cistron translation ratio. CAT protein and luciferase activity values represent the average of at least three independent experiments. Error bars, SD of mean values.