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. 2006 Jul 13;34(12):e84. doi: 10.1093/nar/gkl444

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© 2006 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Examples of genes identified by MS-PET analysis. (A) Novel gene discovery. PET_ID# 48 955.1 (green arrowed line) identifies a novel gene transcript on chromosome 4, and is verified by PCR [inset; F, flanking (primary); N, nested (secondary)] and DNA sequencing of the amplicon (black arrowed lines A07FF and A07FR). (B) Validation of a predicted gene. PET_ID# 282 423.1 (green arrowed line) identifies a predicted gene on chromosome 4, and is verified by PCR [inset; F, flanking (primary); N, nested (secondary)] and DNA sequencing of the amplicon (black arrowed blocks G06FF and G06FR).