Figure 1.

Detection of novel sRNAs by northern analysis. Total RNA was extracted from cultures of P.aeruginosa strain PAO1 grown in LB to exponential phase (first lane in each blot) or stationary phase (second lane in each blot). Blots were hybridized to radiolabeled DNA oligonucleotide probes and then exposed for varying times; thus the relative intensities of the signals do not correspond to the relative abundance of each sRNA. The approximate positions of size standards are shown on the left. Boxes are included to highlight the major species observed in each blot.