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. 2006 Jul 19;34(12):3523–3532. doi: 10.1093/nar/gkl492

Figure 1.

Figure 1

Immunochemical detection of DNA repair intermediates after laser microirradiation. Widefield fluorescence images of HeLa cells are shown. Cells were fixed ∼5 min after laser microirradiation. Arrows mark sites of irradiation. (A) Laser microirradiation with 405 nm results in local generation of DSBs and SSBs detected by antibodies against γ-H2AX and PAR, respectively. Accumulation of endogenous PCNA can be observed at these sites. (B) Both, DNA Ligase I and III accumulate at sites of DNA damage and colocalize with PCNA. (C) XRCC1 and PCNA accumulate at laser-induced DNA damage sites. Scale bars, 5 μm.