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. 2006 Jul 13;34(12):e85. doi: 10.1093/nar/gkl400

Figure 3.

Figure 3

Effect of target position in assessment of PRKR copy number in gDNA and cDNA. (A) Target regions of five primers in PRKR transcript. (B) Absolute quantities in 9 ng of three gDNA (HT, human tonsil; HS, human subcarinal lymph node; HL, human lung) assessed using five primer pairs targeting different regions of the transcript. (C) Absolute quantities in 10 ng of cDNA from IFN treated or untreated U937 human monocytic cell line and 1 ng of cDNA from human peripheral CD4 and CD8 cells assessed using five primer pairs targeting different regions of the transcript. (D) Ratio of PRKR copy numbers between IFN treated and untreated U937 cells, and CD8+ and CD4+ T cells.