Table 1.

Effect of sample complexity on amplification efficiency: standard curves were constructed from a plasmid containing varying copies of the green fluorescent protein gene. The slope, R² value and efficiency of standard curves are shown in the absence of background material and in the presence of increasing quantities of gDNA or CDNA

BDC	Water	gDNA					cDNA
	0	0.111	0.333	1	3	9	1	3	9	27
Slope	−3.82	−3.39	−3.26	−3.20	−3.56	−3.31	−3.43	−3.48	−3.57	−3.36
R2	0.993	0.993	0.989	0.994	0.997	0.992	0.995	0.995	0.995	0.993
Efficiency	83	97	103	105	91	101	96	94	91	98

BDC, background DNA concentration (ng/reaction).