Table 2.

Effect of sample complexity on amplification efficiency: GFP plasmid amplification in presence of varying concentrations of unsheared or sheared gDNA

	BDC	0	30.8	38.6	48	60
Unsheared	Slope	−3.30	−1.74	−1.41	−0.96	−0.48
	R2	0.990	0.865	0.660	0.643	0.176
	Efficiency	101	275	N/A	N/A	N/A
Sheared	Slope	−3.30	−2.97	−3.17	−3.15	−2.78
	R2	0.990	0.994	0.986	0.995	0.980
	Efficiency	101	117	107	108	129

N/A, not available due to poor correlation between replicates.