Table 3.

Effect of sample complexity on amplification efficiency: effect of shearing on amplification of eight target genes

Gene Primer Pair	Slope		R2		Efficiency
	Unsheared	Sheared	Unsheared	Sheared	Unsheared	Sheared
HsIFNB1	−3.36	−3.26	0.978	0.984	99	102
HsTLR3	−3.28	−3.31	0.985	0.979	102	101
HsIRF7	−2.83	−3.06	0.925	0.967	125	112
HsSCYA2	−3.09	−3.35	0.988	0.990	111	99
HsPRKR P2	−3.27	−3.43	0.993	0.996	102	96
HsOAS2	−3.29	−3.25	0.994	0.993	102	103
HsIFIT1	−3.36	−3.36	0.918	0.982	98	99
HsGAPD	−3.43	−3.42	0.981	0.991	96	96

R² indicates the degree of correlation between replicates.

Amplification efficiency (%) = [10^(−1/slope) − 1] × 100.