Figure 1.

(a) Restriction map of the donor plasmid pRTRA, see the text for details. (b) Construction of a recombinant adenoviral genome containing DsRed cassette. The DsRed cassette was transferred to the adenoviral vector by standard MAGIC procedure. The mixed bacteria were selected on LB plates containing 50 μg ml⁻¹ kanamycin,100 μg ml⁻¹ampicillin, 0.2% w/v l-arabinose and 10 mM Cl-Phe overnight. Plasmid DNA was isolated from 14 individual colonies. All plasmid DNAs were digested with SalI and the digestion products were electrophoresed on 0.7% agarose gels and stained with ethidium bromide. Expected restriction patterns for both pAd-DsRed (1–14, for each independently obtained plasmid) and pAd-pheS are shown opposite the gel.