Table 1.
Relationship existing between the X_HYBRID scores and the number of matching nucleotides in Blast hits
| Number of Mismatchs | 0 | 1 | 2 | 3 | 4 |
|---|---|---|---|---|---|
| X_HYBRID = 0 | 14/14 | 17/18 | 20/22 | 23/26 | 26/30 |
| X_HYBRID = 1.x | 16/16 | 19/20 | 22/24 | 25/28 | 28/32 |
| X_HYBRID = 2.x | 18/18 | 21/22 | 24/26 | 27/30 | 30/34 |
| X_HYBRID = 3.x | 20/20 | 22/23 | 25/27 | 28/31 | 31/35 |
| X_HYBRID = 4.x | 21/21 | 24/25 | 27/29 | 30/33 | 33/37 |
| X_HYBRID = 5.x | 23/23 | 26/27 | 29/31 | 31/34 | 35/39 |
| X_HYBRID = 6.x | 41/41 | 44/45 | 47/49 | — | — |
For instance, a probe with a X_HYBRID score equal to 2.1 can match its BLAST hit by 18 identical bases out of 18 (no mismatch), or 21 out of 22 (one mismatch) or 24 out of 26 (two mismatches).