Table 2.
Human | Mouse | |||
---|---|---|---|---|
Probes | Transcripts | Probes | Transcripts | |
Number of distinct RNG/MRC sequences | 148 993 | 29 894 | 121 703 | 26 058 |
Number of distinct gene symbols | — | 21 043 | — | 22 740 |
Total number of RNG/MRC probes | 25 342 | 29 894 | 24 109 | 25 002 |
Number of RNG/MRC probes with x_hyb = 0 | 17 457 | — | 16 767 | — |
Number of RNG/MRC probes with x_hyb = 1,x | 5921 | — | 5563 | — |
Number of RNG/MRC probes with x_hyb > 1,y | 1964 | — | 1779 | — |
Average EST_NUMBER per RNG/MRC probe | 53 | — | 39 | — |
Average DIST_TO_3′score per RNG/MRC probe | 692 | — | 488 | — |
Affymetrix U133Plus2 / MG-U74 | 108 371a | 27 588b | 89 502a | 20 624b |
Agilent whole genome | 40 990 | 25 627b | 20 865 | 18 087b |
Illumina human / MEEBO | 22 548 | 21 271b | 36 362 | 22 463b |
Oligonucleotides with X_HYBRID equal to 0 or to 1,x are considered ‘BLAST-specific’ probes; oligonucleotides with a X_HYBRID equal to x,y (x > 1) can possibly cross-hybridize with other transcripts. They were selected only when no better probe was available for a given transcript. For Affymetrix, comparison was performed for the first and last 25mer probes of each perfect-match Affymetrix probe sets (a probe set is specific to a gene and is composed of an average of ten 25mer probes). A total of 90% of the transcripts have a majority of their associated oligonucleotides characterized by a X_HYBRID score below 2. Less than 5% of the transcripts are only associated with oligonucleotides characterized by a X_HYBRID score above 2.
aOnly the first and last probes from each Affymetrix probe set were used for analysis.
bNumber of transcripts matched by probes from the RNG/MRC transcript selection set.