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. 2006 Jul 19;34(12):e88. doi: 10.1093/nar/gkl461

Figure 2.

Figure 2

Identification of novel paternally expressed transcripts on mouse proximal Chr 7. (A) The 2 Mb PWS/AS orthologous region with positions of the novel paternally expressed transcripts indicated*. Genes are shown as open boxes with the relative transcriptional orientations defined by arrows. Blue, red or no colouring define paternal, maternal or biallelic expression respectively. (B) RT–PCR analysis of candidates identified on the proximal Chr 7 brain array in cDNA derived from patDp prox 7, matDp prox 7 and wild-type brain tissue. Controls for paternal (Snrpn), maternal (Grb10) and biallelic (Igf1r) expression are shown. The molecular weight marker is a 100 bp DNA ladder where the bright band corresponds to 500 bp. Samples treated with and without reverse transcriptase are indicated as + or −RT. ESTs AK080843, BB077283, BM117114 and AV328498 were paternally expressed. (C) Allele-specific RT–PCR analysis of proximal Chr 7 candidates. Newborn brain tissues with expressed SNPs were obtained from reciprocal crosses between M.m.musculus (B6) and M.m.castaneus (CAST) animals. cDNA fragments containing the SNPs were recovered by RT–PCR and direct sequencing to determine allele-specific expression.