Fig. 2.
Activation marker expression on and effector function of the proliferating P14 T cells are unaffected by competition. (A and B) Experiments were performed similarly to Fig. 1A, and CFSE intensity was plotted against CD122 (A) or intracellular IFN-γ (B). The mean fluorescence intensity (MFI) for CD122 expression in cells that have completely lost CFSE staining (region shown on dot plot) is noted in A. Histograms are shown for intracellular IFN-γ expression in fully divided P14 cells in B. Mice were immunized with 2 × 106 DCs. (C) CFSE-labeled P14.Thy1.1 cells and OT-I cells were transferred into mice and immunized with unpulsed or peptide-pulsed DCs as shown, and spleen cells were tested for CD44 and Bcl-XL expression at 44–48 h. MFI for CD44 and Bcl-XL are plotted for undivided cells and cells in rounds 1–4 of division. ■, P14 alone; ●, P14 + OT-I.