TABLE 1.

PCR primers used in this work

Forward and reverse primers	Sequence 5′-3′	Amplicon (size in bp)	Position	Remark	Reference
int1f imp12	CAG AAG ACG GCT GCA CTG AA AGT GTG TCC TGG GCC TGG	blaIMP-4 upstream (1,833)	−1359 to +474 (blaIMP-4 ATG)	Characterization of the upstream region of blaIMP-4 in strain 74510	This work
imp11 int3c	ATG AGC AAA GTT ATC TGT ATT CT AAA GCA GTC TTG ACC TGA	blaIMP-4 downstream (2,812)	+741 (blaIMP-4 ATG) to 2071 downstream of blaIMP-4	Characterization of the downstream region of blaIMP-4 in strain 74510	This work
int2a int6b	AAC CGA GGA TGC GAA CCA CT CCG AGC CGC TCG TAT AG	intI1-arr-2-aacA4-3′CS (3,602)	+382 (intI1 ATG) to +482 (orf5 ATG)	Characterization of the regions flanking arr-2 in strain 74510	This work
int2a imp12	AAC CGA GGA TGC GAA CCA CT AGT GTG TCC TGG GCC TGG	intI1-blaIMP-4 (1,008)	+382 (intI1 ATG) to + 474 (blaIMP-4 ATG)	Combining with RFLP for detection of the intI1-blaIMP-4 sequencea	This work
int1b aac61bB	CAT CCA AGC AGC AAG CGC GTT A ACC CCG GIT TCT CGT AGC AT	5′ CS-arr-2-aacA4 (1,182)	−131 (arr-2 ATG) to +460 (aacA4 ATG)	Combining with RFLP for the detection of the 5′ CS-arr-2-aacA4 sequencea	This work
int1e int1c	TCG TAG AGA CGT CGG AAT GG CCG AGG CAT AGA CTG TAC AA	Class 1 integrase gene intI1 (965)	−40 to +925 (intI1 ATG)	For detection of the intI1 gene	27
5cs 3cs	GGC ATC CAA GCA GCA AG AAA GCA GAC TTG ACC TGA	Entire integron (variable)	Variable	For confirmation of the presence of class 1 integron	27
ardra1 ardra2	TGG CTC AGA TTG AAC GCT TAC CTG TTA CGA CTT CA	16S rRNA gene (1,500)	Variable	ARDRA of acinetobacter for genomic DNA group determination	11
BL BR	TAT GAG TGG CTA AAT CGA T CCC GCT TTC TCG TAG CA	aacA4 (395)	+115 to +509 (aacA4 ATG)	For detection of the aacA4 gene	39
aph1 aph2	ATA CAG AGA CCA CCA TAC AGT GGA CAA TCA ATA ATA GCA AT	aph(3′)-VIa (234)	+140 to +374 [aph(3′)-VIa ATG]	For detection of the aph(3′)-VIa gene	60

^aSee Table 2 for the expected sizes of the restriction fragments.