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Expected fragments in the PCR-RFLP screening for the intI1-blaIMP-4 and 5′ CS-arr-2-aacA4 sequences
| Restriction enzyme | Expected fragments (bp) |
|---|---|
| For detection of the intI1-blaIMP-4 (1,008-bp) sequence | |
| ApaLI | 96, 912 |
| BsmAI | 157, 851 |
| HinCII | 57, 268, 288, 395 |
| HindIII | 292, 716 |
| For detection of the 5′CS-arr-2-aacA4 (1,182-bp) sequence | |
| BsoBI | 73, 350, 759 |
| BspHI | 160, 599, 423 |
