Figure 3.

Silencing of the luciferase gene by siRNAs at different concentrations. Protoplasts were prepared from 2-week-old kitaake plants and transformed as described (Methods). Varying concentrations of siRNA to luciferase (0 μg, 0.3 μg, 1 μg, 2 μg, 3 μg, 5 μg, 6 μg and 10 μg) were transformed into protoplasts along with pLUC (10 μg) and pGUS (10 μg) as an internal control. Relative luciferase activity values for each concentration of siRNA were normalized to the observed GUS expression from the same set of protoplasts. Two independent experiments (separate preparations of protoplasts) were performed with similar outcomes. Each experiment contained two replicates for each concentration of siRNAs.