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. 2003 Apr;47(4):1200–1206. doi: 10.1128/AAC.47.4.1200-1206.2003

FIG. 2.

FIG. 2.

(a) Plate counts (numbers of CFU per milliliter) and particle counts per milliliter for C. albicans strain MY2417 incubated with AMB for 10 h at 35°C, followed by incubation for 15 h at 22°C or by incubation with miconazole at 20 μg/ml for 15 h at 22°C. ○, numbers of CFU per milliliter after incubation for 10 h at 35°C, followed by incubation for 15 h at 22°C; □, particle counts per milliliter after incubation for 10 h at 35°C, followed by incubation for 15 h at 22°C; •, numbers of CFU per milliliter after incubation for 10 h at 35°C, followed by incubation with miconazole at 20 μg/ml for 15 h at 22°C; ▪, particle counts per milliliter after incubation for 10 h at 35°C, followed by incubation for 15 h at 22°C with miconazole at 20 μg/ml; →, numbers of CFU per milliliter and particle counts per milliliter at time zero. Error bars indicate standard errors. (b) DiBAC4(3), SYBR Green Ι, and CFDA staining of C. albicans strain MY2417 incubated with AMB for 10 h at 35°C, followed by incubation for 15 h at 22°C or with miconazole at 20 μg/ml for 15 h at 22°C. ▾ and a▿, DiBAC4(3); ▴ and ▵, SYBR Green Ι; ▪ and □, CFDA; closed symbols, incubation for 10 h at 35°C, followed by incubation with miconazole at 20 μg/ml for 15 h at 22°C; open symbols, incubation for 10 h at 35°C, followed by incubation for 15 h at 22°C; →, fluorescent staining values at time zero. Error bars indicate standard errors. (c) Intracellular ATP concentration for C. albicans strain MY2417 incubated with AMB for 10 h at 35°C, followed by incubation for 15 h at 22°C or incubation with miconazole at 20 μg/ml for 15 h at 22°C. □, incubation for 10 h at 35°C, followed by incubation for 15 h at 22°C; ▪, incubation for 10 h at 35°C, followed by incubation with miconazole at 20 μg/ml for 15 h at 22°C; →, intracellular ATP concentration at time zero. Error bars indicate standard errors. (d) XTT reduction for C. albicans strain MY2417 incubated with AMB for 10 h at 35°C, followed by incubation for 15 h at 22°C or by incubation with miconazole at 20 μg/ml for 15 h at 22°C. □, incubation for 10 h at 35°C, followed by incubation for 15 h at 22°C; ▪, incubation for 10 h at 35°C, followed by incubation with miconazole at 20 μg/ml for 15 h at 22°C; →, XTT reduction at time zero. Error bars indicate standard errors.