Figure 2.

Western blotting (confirmed from three separate experiments) of human mammary epithelial cells (HMEC) from donor 4 at various stages from mortal, early passage to the epithelial tumor are shown below. Volume intensity of H-ras is displayed on the bar graph at the top, and H-ras overexpression compared to the baseline mortal state is indicated below each bar. Lanes (from right to left): 1. HMEC from donor 4- post stasis, pre agonescence, passage 9. 2. RAO-1 cells. 3. RAO-2 cells. 4. Cells from soft agar colony that developed into the epithelial tumor in nude mice. 5. RAO-4 cells, cultured in monolayer